Purpose: We studied the pathogens and trends in antibiotic sensitivity pattern in children with urinary tract infection(UTI) over 8 years in order to adequate treatment. Methods: We performed a retrospective review of medical records of children with UTI from January 2009 to December 2016 in Daegu Fatima Hospital. Uropathogens and antibiotic sensitivity pattern are selected. Only 1 bacterial specie with a colony count of ≥105 CFU/mL is considered positive result. We compared 2 periods group (A: 2009~2012, B: 2013~2016) to investigate trends of antibiotic sensitivity pattern. Results: During 8 years, 589 cases are collected (509 cases was cultured E.coli , 86.4%). Antimicrobial susceptibility to ampicillin was steadily low for 2 periods (A: 31.6%, B: 37.6%, P=0.176). Antimicrobial susceptibility to amikacin was consistently high for 2 periods (A: 99.2%, B: 99.4%, P=1.000). Antibiotic sensitivity to 3rd cephalosporin was decreased from period A to B (A: 91.5%, B: 77.8%, P=0.000). The prevelance of Extended-spectrum β-lactamase(ESBL) producing E.coli was increased from period A to B (A: 6.2%, B: 17.4%, P=0.000). Conclusion: This study showed conventional antibiotic therapy for the treatment of pediatric UTI needs to be reevaluated. A careful antibiotic choice is required due to the change of antibiotic sensitivity and the emergence of antibiotic resistant bacteria.